Fisher BioReagents

 

Fisher BioReagents: Your Source For High-Purity Products

Fisher BioReagentsThe Fisher BioReagents brand delivers Convenience, Quality and Consistency.

We offer essential reagents pre-qualified for dedicated applications, eliminating the need for testing. Our high-purity products are designed for a range of applications in the areas of:

Molecular Biology

Protein Chemistry

Cell Biology

What's New:

NEW OPTIZYME* DNA/RNA Modifying Enzymes

OPTIZYME DNA/RNA Modifying Enzymes are manufactured using high yielding expression vectors in class D clean rooms under strict ISO9001 quality and ISO14001 environmental management systems. By combining leading recombinant production technology with value pricing and batch-to-batch consistency, OPTIZYME customers benefit from both substantial savings and improved quality and consistency on modifying enzymes used for:

  • DNA cloning
  • In vitro transcription
  • cDNA synthesis

Key features:

  • Quality assurance tested for functional purity and activity
  • Nuclease-free
  • Value pricing

See products available»

NEW OPTIZYME* Restriction Enzymes

Genomics applications, such as gene cloning and DNA genotyping, require high-quality restriction enzymes. The Fisher BioReagents OPTIZYME line of restriction enzymes offers customers an outstanding combination of quality, performance and low prices.

Outstanding performance and convenience:

  • Economy: Competitively priced
  • Quality: Outstanding quality and consistency
  • Selection: A broad range of the most commonly used restriction enzymes
  • Packaging: Product specifications are presented in a user-friendly and convenient format

See products available»

NEW Bovine Serum Albumin (BSA) Products from Fisher BioReagents

Key features:

  • Certified U.S. origin
  • Includes low endotoxin, protease-free and other application-specific BSA grades
  • Meets rigorous quality control tests including purity, solubility and heavy metal content
  • Suitable for use as growth supplement in various cell culture systems, such as serum-free and microbiological media

Cat. No.

Product Description

Pack Size

BP9700-100

BSA Microbiological Grade Powder, purified by a modified cold ethanol procedure

100g

BP9703-100

BSA Protease-Free Powder, purified by a heat shock process

100g

BP9704-100

BSA Fatty Acid-Free Powder, purified by a heat shock process with additional treatments

100g

BP9705-100

BSA Low Endotoxin Powder, purified by a heat shock process with additional treatments

100g

BP9706-100

BSA DNase- and Protease-Free Powder, purified by proprietary heat shock process

100g


 

Water Microbial Cell Culture GradeWater, Microbial Cell Culture Grade

Ultrapure water for preparing microbiological growth media

  • 0.03 micron filtered and autoclaved to ensure high purity
  • Deionized for very low metal ion content
  • Low level of endotoxin <0.25 EU/mL
  • Total organic carbon (TOC) <50ppb
  • Functionally tested for promoting microbial growth

Applications

  • Preparing media for microbial growth and maintenance
    • Lambda Phage (2XYT Broth, NZCYM Broth)
    • E. coli (LB Broth, LB Agar)
    • Yeast (YPD Broth)
  • Preparing media for plating
    • M13 Phage (2XYT Agar, NZCYM Agar)
    • Preparing media for cloning (transformation)
    • E. coli (SOC Medium, SOB Medium, Terrific Broth)
  • Preparing antibiotic solutions

See available pack sizes »

 

Media for Competent Cells in Standard Cloning Applications
Bacteriological media especially useful for DHα strains of E. coli.

  • Propagation of bacteria in suspension, especially DHα strains and TransMax Competent Cells 
  • SOB Broth formulation per liter: casein peptone 20g, yeast extract 5g, sodium chloride 0.5g, magnesium sulfate 2.4g, and potassium chloride 0.186g
  • SOC Medium formulation per liter: casein peptone 20g, yeast extract 5g, sodium chloride 0.58g, potassium chloride 0.19g, magnesium chloride 0.95g, magnesium sulfate 1.2g, and glucose 3.6g
  • Developed for use in high efficiency plasmid DNA transformations

 Description

 Cat. No.

SOB Broth, Capsules, 36 x 14g capsules

BP9737-500

SOC Medium

BP9740-10X5

2M Magnesium Chloride, Microbial Cell Culture Grade

BP9741-10X5

2M Calcium Chloride, Microbial Cell Culture Grade

BP9742-10X5

CellPure Buffers

CellPURE Biological Buffers

These ultrapure zwitterionic buffers are optimized for cell culture work. They can be used in a wide variety of molecular biology and biochemical studies. Strict manufacturing controls for quality ensure performance and reliability.

Key features:

  • Cell culture tested
  • Analyzed for the absence of nucleases and proteases
  • Tested for endotoxin and bioburden levels
  • pKa values mostly independent of temperature and concentration
  • High water solubility
  • Minimal permeability to biological membranes

Available CellPure Buffers »

JustPURE Buffers

JustPURE “Good” Buffers

An ultrapure zwitterionic buffer that is optimized for use in cellular and molecular biology, JustPURE "Good" Buffers feature low interference with biological reactions.

Key Features:

  • pKa values mostly independent of temperature and concentration
  • High water solubility
  • Chemically and enzymatically stable
  • Minimal permeability to biological membranes
  • Limited effect on biochemical reactions
  • Minimal absorption in spectral range 240-700nm

Available JustPure Buffers »

 

Isopropanol, Molecular Biology Grade

High Purity Isopropanol (IPA) is a staple reagent chemical used in many life science laboratories. Fisher BioReagents has developed a new ultrapure Isopropanol, Molecular Biology Grade that can be used in fundamental applications, such as purification and precipitation of nucleic acids and proteins and preservation of biological specimens.

Key features:

  1. Ultrapure Isopropanol (assay >99.9 percent) for molecular biology work
  2. Tested for DNase, RNase and Protease to ensure absence of these hydrolyzing enzymes
  3. Water <0.05 percent
  4. Low UV optical absorbance
  5. Variety of product pack sizes to meet various laboratory needs
  6. Meets the Optima* specifications for ultrapure isopropanol

View additional product information » (PDF, 593KB)

Purchase Isopropanol, Molecular Biology Grade »

 

Antibiotics and Antimycotics15 New Antibiotics and Antimycotics

  • High quality – antibiotics and antimycotics meet rigorous quality control tests, including verification of potency, purity and solubility using microbiological and chromatographic methods.
  • Built for selection – excellent choice of antibiotics for selection of antibiotic resistance genes in both bacterial and mammalian cells.
  • High purity and stability –- ultrapure antibiotics ensure successful growth of target cells and reproducible results.
  • Maximum convenience – antibiotics and antimycotics come in both sterile powder and liquid forms.

View the full line of Fisher BioReagents Antibiotics and Antimycotics »

 

Fisher BioReagents Water, Molecular Biology GradeFisher BioReagents Water (0.1 Micron Filtered), Molecular Biology Grade

In the life sciences, high purity water is a staple solvent used in all cell and molecular biology laboratories. Water, Molecular Biology Grade is ideal for many fundamental procedures such as PCR, electrophoresis, DNA sequencing and buffers for enzymatic analyses.

Key features:

  1. 0.1 micron filtered to ensure high purity
  2. Tested for DNase, RNase and Protease to ensure absence of these hydrolytic enzymes
  3. Deionized for very low metal ion content
  4. Available in a variety of product pack sizes to meet various laboratory needs

Applications:

  1. Ideal for making reagents, rinsing glass and plastic ware and sample preparation
  2. DNA/RNA/Protein extraction and purification
  3. Used to prepare buffers for enzymatic reactions
  4. PCR technology, blotting applications and DNA Sequencing

Purchase Fisher BioReagents Water (0.1 Micron Filtered), Molecular Biology Grade »

 

Fisher BioReagents Absolute Ethanol

Fisher BioReagents Absolute Ethanol (200 Proof), Molecular Biology Grade

Fisher BioReagents Absolute Ethanol (200 proof) is an ultrapure molecular biology grade ethanol used for the purification and precipitation of biomolecules such as nucleic acids and proteins.

Key features:

  1. 200 proof, absolute ethyl alcohol
  2. Tested for DNase, RNase and Protease to ensure absence of these hydrolyzing enzymes
  3. Meets ACS specifications for Absolute Ethyl Alcohol
  4. All federal taxes paid
  5. 0.2 micron filtered
  6. Water ≤0.2 percent
  7. Variety of product pack sizes to meet various laboratory needs

Applications:

  1. Purification and precipitation of nucleic acids (DNA and RNA) and proteins
  2. Preparation of staining and destaining solutions
  3. Dehydration of cells and tissues prior to paraffin wax embedding
  4. Extraction medium
  5. Chromatographic reagent

Purchase Fisher BioReagents Absolute Ethanol »

 

Fisher BioReagents Purity Grades

 

View the Fisher BioReagents purity grade chart » (PDF, 192KB)